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Preliminary study on preparation of cell-free system for protein expression

CHEN Haiqin, XU Zhinan, WANG Cheng, CEN Peilin, LIAO Yuhua

Frontiers of Chemical Science and Engineering 2008, Volume 2, Issue 2,   Pages 224-229 doi: 10.1007/s11705-008-0029-9

Abstract: The cell-free protein synthesis system provides a new strategy of protein expression with advantagessystem are the key factors for the successful construction of cell-free protein expression system.The cell growth phase, the pressure for cell disruption and the storage condition of cell extracts wereUnder the optimized conditions, the green fluorescent protein (GFP) reporter gene was expressed in the cell-freesystem with high expression level (Ca. 154 ?

Keywords： cell-free     regeneration     extract     information     successful construction    

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Cell-free systems in the new age of synthetic biology

Fernando Villarreal,Cheemeng Tan

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 58-65 doi: 10.1007/s11705-017-1610-x

Abstract: The advent of synthetic biology has ushered in new applications of cell-free transcription-translationThese cell-free systems are reconstituted using cellular proteins, and are amenable to modular controlHere, we discuss the historical advancement of cell-free systems, as well as their new applications inFinally, we present our vision on the future direction of cell-free synthetic biology.

Keywords： cell-free system     application    

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Application of liquid biopsy in precision medicine: opportunities and challenges

Junyun Wang, Shuang Chang, Guochao Li, Yingli Sun

Frontiers of Medicine 2017, Volume 11, Issue 4,   Pages 522-527 doi: 10.1007/s11684-017-0526-7

Abstract: Detecting circulating tumor cell (CTC) or circulating tumor DNA (ctDNA) in plasma or serum could serve

Keywords： liquid biopsy     circulating tumor cells     cell-free ctDNA     exosomes     precision medicine    

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Microcystin-LR biodegradation by

Chengbin XIAO, Hai YAN, Junfeng WANG, Wei WEI, Jun NING, Gang PAN

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 4,   Pages 526-532 doi: 10.1007/s11783-010-0261-7

Abstract: cyanobacterial cells collected from Lake Guishui and the biodegradation of MC-LR by both strain and cell-free

Keywords： microcystin-LR(MC-LR)     biodegradation     Sphingopyxis sp     USTB-05     cell-free extract    

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Enhanced Poly(ethylene terephthalate) Hydrolase Activity by Protein Engineering Article

Yuan Ma, Mingdong Yao, Bingzhi Li, Mingzhu Ding, Bo He, Si Chen, Xiao Zhou, Yingjin Yuan

Engineering 2018, Volume 4, Issue 6,   Pages 888-893 doi: 10.1016/j.eng.2018.09.007

Abstract: The enzymatic activities of the R61A, L88F, and I179F mutants, which were obtained with a rapid cell-freescreening system, exhibited 1.4 fold, 2.1 fold, and 2.5 fold increases, respectively, in comparison

Keywords： Polyesterase     PET degradation     Cell-free protein synthesis     Polyester     PETase    

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MEC-Empowered Non-Terrestrial Network for 6G Wide-Area Time-Sensitive Internet of Things Article

Chengxiao Liu, Wei Feng, Xiaoming Tao, Ning Ge

Engineering 2022, Volume 8, Issue 1,   Pages 96-107 doi: 10.1016/j.eng.2021.11.002

Abstract: Thus, we can build a non-terrestrial network (NTN) using a cell-free architecture.

Keywords： Cell-free Mobile edge computing     Non-terrestrial networks     Sixth-generation     Wide-area time-sensitive    

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A space power system of free piston Stirling generator based on potassium heat pipe

Mingqiang LIN, Jian MOU, Chunyun CHI, Guotong HONG, Panhe GE, Gu HU

Frontiers in Energy 2020, Volume 14, Issue 1,   Pages 1-10 doi: 10.1007/s11708-019-0655-6

Abstract: The power system of a free piston Stirling generator (FPSG) based on potassium heat pipes has been developedBesides, the power system integrated with four FPSGs and potassium heat pipes was performed and the singleThe results show that this system could provide an electrical power of 300 W at an overall thermal efficiencyThus, it is concluded that this power system is feasible and will have a great prospect for future applications

Keywords： free piston Stirling generator (FPSG)     potassium heat pipe     power system     energy conversion    

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An extended cell-based smoothed discrete shear gap method (XCS-FEM-DSG3) for free vibration analysis

M. H. NGUYEN-THOI,L. Le-ANH,V. Ho-HUU,H. Dang-TRUNG,T. NGUYEN-THOI

Frontiers of Structural and Civil Engineering 2015, Volume 9, Issue 4,   Pages 341-358 doi: 10.1007/s11709-015-0302-1

Abstract: A cell-based smoothed discrete shear gap method (CS-FEM-DSG3) was recently proposed and proven to berobust for free vibration analyses of Reissner-Mindlin shell.The method improves significantly the accuracy of the solution due to softening effect of the cell-basedThe paper hence tries to extend the CS-FEM-DSG3 for free vibration analysis of cracked Reissner-MindlinThe accuracy and reliability of the novel XCS-FEM-DSG3 for free vibration analysis of cracked Reissner-Mindlin

Keywords： cracked Reissner-Mindlin shell     free vibration analysis     cell-based smoothed discrete shear gap method(CS-FEM-DSG3)     extended cell-based smoothed discrete shear gap method (XCS-FEM-DSG3)     smoothed finite element    

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NADPH oxidase and reactive oxygen species as signaling molecules in carcinogenesis

Gang WANG

Frontiers of Medicine 2009, Volume 3, Issue 1,   Pages 1-7 doi: 10.1007/s11684-009-0018-5

Abstract: Reactive oxygen species (ROS) are small molecule metabolites of oxygen that are prone to participate in redox reactions their high reactivity. Intracellular ROS could be generated in reduced nicotinamide-adenine dinucleotidephosphate (NADPH) oxidase-dependent and/or NADPH oxidase-independent manners. Physiologically, ROS are involved in many signaling cascades that contribute to normal processes. One classical example is that ROS derived from the NADPH oxidase and released in neurotrophils are able to digest invading bacteria. Excessive ROS, however, contribute to pathogenesis of various human diseases including cancer, aging, dimentia and hypertension. As signaling messengers, ROS are able to oxidize many targets such as DNA, proteins and lipids, which may be linked with tumor growth, invasion or metastasis. The present review summarizes recent advances in our comprehensive understanding of ROS-linked signaling pathways in regulation of tumor growth, invasion and metastasis, and focuses on the role of the NADPH oxidase-derived ROS in cancer pathogenesis.

Keywords： free radicals     tumor     phox     cell proliferation     cancer therapy    

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Design and modeling of a free-piston engine generator

Frontiers in Energy   Pages 811-821 doi: 10.1007/s11708-022-0848-2

Abstract: Free-piston engine generators (FPEGs) can be applied as decarbonized range extenders for electric vehiclesThe designed FPEG produced 10.2 kW of electric power with an overall system efficiency of 38.5% in a

Keywords： free-piston engine generator     linear permanent magnet synchronous machine     system design     numerical model    

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Comparison of reference values for immune recovery between event-free patients receiving haploidentical

Xuying Pei, Xiangyu Zhao, Yu Wang, Lanping Xu, Xiaohui Zhang, Kaiyan Liu, Yingjun Chang, Xiaojun Huang

Frontiers of Medicine 2018, Volume 12, Issue 2,   Pages 153-163 doi: 10.1007/s11684-017-0548-1

Abstract:

To establish optimal reference values for recovered immune cell subsets, we prospectively investigatedpost-transplant immune reconstitution (IR) in 144 patients who received allogeneic stem cell transplantationthat data from this recipient cohort should be used as reference values for post-transplant immune cell

Keywords： immune reconstitution     hematopoietic stem cell transplantation     event-free patients     reference range    

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The ecological adaptability of cloned sheep to free-grazing in the Tengger Desert of Inner Mongolia,

Xinxin LI,Huijuan WANG,Guanghua SU,Zhuying WEI,Chunling BAI,Wuni-MENGHE,Yanhui HOU,Changqing YU,Shorgan BOU,Guangpeng LI

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3,   Pages 191-200 doi: 10.15302/J-FASE-2014029

Abstract: Since the birth of the first cloned sheep, somatic cell nuclear transfer technology has been successfullyIn the present study, the cloned Dorper sheep equipped with GPS location devices were free-grazed inThe main findings of this research were as follows. (1) Under free-grazing conditions, the cloned sheepsheep. (3) The cloned sheep exhibited free-grazing patterns and social behavior as other sheep. (4)The Dorper sheep readily adapted to the free-grazing conditions on the Mongolian plateau grassland, which

Keywords： somatic cell nuclear transfer     free-grazing synchronization     Dorper sheep     cloned animal ecology    

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Risk stratification system for skin and soft tissue infections after allogeneic hematopoietic stem cell

Frontiers of Medicine 2022, Volume 16, Issue 6,   Pages 957-968 doi: 10.1007/s11684-021-0910-1

Abstract: However, to date, the profiles and clinical outcomes of SSTIs in hematopoietic stem cell transplantationA PPGF–AKI–HAP risk stratification system was established with a mortality risk score of 1×PPGF+1×AKI

Keywords： skin and soft tissue infections     hematopoietic stem cell transplantation     risk stratification system    

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Piezoelectric pump with flexible venous valves for active cell transmission

Frontiers of Mechanical Engineering 2022, Volume 17, Issue 4, doi: 10.1007/s11465-022-0712-4

Abstract: Cell survival rate can reach 97.22% with a yeast cell culture solution as the transmission medium.The proposed pump can help to construct the circulating organ-on-a-chip system, and the simple structurefacilitating the optimal design of the micro-pump and providing a reference for the further study of active cell

Keywords： venous valve     flexible venous valve     cell transmission     organ-on-a-chip system     piezoelectric device    

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Nitrogen-doped graphene approach to enhance the performance of a membraneless enzymatic biofuel cell

Alireza AHMADIAN YAZDI, Jie XU

Frontiers in Energy 2018, Volume 12, Issue 2,   Pages 233-238 doi: 10.1007/s11708-018-0529-3

Abstract: the anodic response to glucose detection leads to a higher overall performance of enzymatic biofuel cellThus, here we first synthesized N-graphene via a catalyst-free single-step thermal process, and made

Keywords： enzymatic fuel cell     nitrogen-doped graphene     reduced graphene oxide     catalyst-free synthesis    

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Title Author Date Type Operation

Preliminary study on preparation of cell-free system for protein expression

CHEN Haiqin, XU Zhinan, WANG Cheng, CEN Peilin, LIAO Yuhua

Journal Article

Cell-free systems in the new age of synthetic biology

Fernando Villarreal,Cheemeng Tan

Journal Article

Application of liquid biopsy in precision medicine: opportunities and challenges

Junyun Wang, Shuang Chang, Guochao Li, Yingli Sun

Journal Article

Microcystin-LR biodegradation by

Chengbin XIAO, Hai YAN, Junfeng WANG, Wei WEI, Jun NING, Gang PAN

Journal Article

Enhanced Poly(ethylene terephthalate) Hydrolase Activity by Protein Engineering

Yuan Ma, Mingdong Yao, Bingzhi Li, Mingzhu Ding, Bo He, Si Chen, Xiao Zhou, Yingjin Yuan

Journal Article

MEC-Empowered Non-Terrestrial Network for 6G Wide-Area Time-Sensitive Internet of Things

Chengxiao Liu, Wei Feng, Xiaoming Tao, Ning Ge

Journal Article

A space power system of free piston Stirling generator based on potassium heat pipe

Mingqiang LIN, Jian MOU, Chunyun CHI, Guotong HONG, Panhe GE, Gu HU

Journal Article

An extended cell-based smoothed discrete shear gap method (XCS-FEM-DSG3) for free vibration analysis

M. H. NGUYEN-THOI,L. Le-ANH,V. Ho-HUU,H. Dang-TRUNG,T. NGUYEN-THOI

Journal Article

NADPH oxidase and reactive oxygen species as signaling molecules in carcinogenesis

Gang WANG

Journal Article

Design and modeling of a free-piston engine generator

Journal Article

Comparison of reference values for immune recovery between event-free patients receiving haploidentical

Xuying Pei, Xiangyu Zhao, Yu Wang, Lanping Xu, Xiaohui Zhang, Kaiyan Liu, Yingjun Chang, Xiaojun Huang

Journal Article

The ecological adaptability of cloned sheep to free-grazing in the Tengger Desert of Inner Mongolia,

Xinxin LI,Huijuan WANG,Guanghua SU,Zhuying WEI,Chunling BAI,Wuni-MENGHE,Yanhui HOU,Changqing YU,Shorgan BOU,Guangpeng LI

Journal Article

Risk stratification system for skin and soft tissue infections after allogeneic hematopoietic stem cell

Journal Article

Piezoelectric pump with flexible venous valves for active cell transmission

Journal Article

Nitrogen-doped graphene approach to enhance the performance of a membraneless enzymatic biofuel cell

Alireza AHMADIAN YAZDI, Jie XU

Journal Article